Calculate sample volumes from BCA absorbance readings instantly. Get accurate protein loading volumes for western blots, enzyme assays, and IP experiments.
Calculate precise sample volumes from BCA absorbance readings and target protein mass. Enter absorbance values and desired protein amounts to get exact volumes for consistent loading.
The sample volume is calculated using the following formula:
ā¢ Keep absorbance between 0.1-2.0 for accurate results within the linear range
ā¢ Typical amounts: 20-50 Ī¼g for western blots, 500-1000 Ī¼g for immunoprecipitation
ā¢ Volumes above 1000 Ī¼L indicate low protein concentrationāconsider concentrating your sample
ā¢ Standard BCA works for most applications (20-2000 Ī¼g/mL). Use Enhanced for dilute samples (5-250 Ī¼g/mL)
Discover more tools that might be useful for your workflow