Calculate qPCR efficiency from Ct values and standard curves. Free tool for PCR amplification efficiency analysis, slope calculation, and assay validation with instant results.
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Value must be positive
Value must be positive
Value must be positive
Value must be positive
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qPCR efficiency is a measure of how well the PCR reaction performs. An efficiency of 100% means that the amount of PCR product doubles with each cycle during the exponential phase.
The efficiency is calculated from the slope of the standard curve, which is obtained by plotting the Ct values against the logarithm of the initial template concentration (dilution series).
The efficiency (E) is calculated using the formula:
E = 10^(-1/slope) - 1
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