DNA 연결 계산기 - 분자 클로닝을 위한 삽입물:벡터 비율 계산

1def calculate_ligation_volumes(vector_concentration, vector_length, insert_concentration, 
2                              insert_length, molar_ratio, total_volume, vector_amount=50):
3    """
4    DNA 리게이션 반응을 위한 볼륨 계산.
5    
6    매개변수:
7    vector_concentration (float): 벡터 DNA 농도 (ng/μL)
8    vector_length (float): 벡터 DNA 길이 (염기쌍)
9    insert_concentration (float): 인서트 DNA 농도 (ng/μL)
10    insert_length (float): 인서트 DNA 길이 (염기쌍)
11    molar_ratio (float): 인서트:벡터의 원하는 몰 비율
12    total_volume (float): 총 반응 볼륨 (μL)
13    vector_amount (float): 사용할 벡터 DNA 양 (ng, 기본값: 50)
14    
15    반환:
16    dict: 계산된 볼륨 및 양을 포함하는 사전
17    """
18    # 벡터 볼륨 계산
19    vector_volume = vector_amount / vector_concentration
20    
21    # 필요한 인서트 양 계산
22    vector_length_kb = vector_length / 1000
23    insert_length_kb = insert_length / 1000
24    insert_amount = (vector_amount * insert_length_kb / vector_length_kb) * molar_ratio
25    
26    # 인서트 볼륨 계산
27    insert_volume = insert_amount / insert_concentration
28    
29    # 버퍼/물 볼륨 계산
30    buffer_volume = total_volume - vector_volume - insert_volume
31    
32    return {
33        "vector_volume": round(vector_volume, 2),
34        "insert_volume": round(insert_volume, 2),
35        "buffer_volume": round(buffer_volume, 2),
36        "insert_amount": round(insert_amount, 2),
37        "vector_amount": vector_amount
38    }
39
40# 사용 예시
41result = calculate_ligation_volumes(
42    vector_concentration=50,
43    vector_length=3000,
44    insert_concentration=25,
45    insert_length=1000,
46    molar_ratio=3,
47    total_volume=20
48)
49
50print(f"벡터: {result['vector_volume']} μL ({result['vector_amount']} ng)")
51print(f"인서트: {result['insert_volume']} μL ({result['insert_amount']} ng)")
52print(f"버퍼: {result['buffer_volume']} μL")
53print(f"총량: 20 μL")
54

1function calculateLigationVolumes(vectorConcentration, vectorLength, insertConcentration, 
2                                insertLength, molarRatio, totalVolume, vectorAmount = 50) {
3  // 길이를 kb로 변환하여 계산
4  const vectorLengthKb = vectorLength / 1000;
5  const insertLengthKb = insertLength / 1000;
6  
7  // 필요한 인서트 양 계산
8  const insertAmount = (vectorAmount * insertLengthKb / vectorLengthKb) * molarRatio;
9  
10  // 볼륨 계산
11  const vectorVolume = vectorAmount / vectorConcentration;
12  const insertVolume = insertAmount / insertConcentration;
13  const bufferVolume = totalVolume - vectorVolume - insertVolume;
14  
15  return {
16    vectorVolume: parseFloat(vectorVolume.toFixed(2)),
17    insertVolume: parseFloat(insertVolume.toFixed(2)),
18    bufferVolume: parseFloat(bufferVolume.toFixed(2)),
19    insertAmount: parseFloat(insertAmount.toFixed(2)),
20    vectorAmount: vectorAmount
21  };
22}
23
24// 사용 예시
25const result = calculateLigationVolumes(50, 3000, 25, 1000, 3, 20);
26console.log(`벡터: ${result.vectorVolume} μL (${result.vectorAmount} ng)`);
27console.log(`인서트: ${result.insertVolume} μL (${result.insertAmount} ng)`);
28console.log(`버퍼: ${result.bufferVolume} μL`);
29console.log(`총량: 20 μL`);
30

1public class DNALigationCalculator {
2    public static class LigationResult {
3        public final double vectorVolume;
4        public final double insertVolume;
5        public final double bufferVolume;
6        public final double insertAmount;
7        public final double vectorAmount;
8        
9        public LigationResult(double vectorVolume, double insertVolume, double bufferVolume, 
10                             double insertAmount, double vectorAmount) {
11            this.vectorVolume = vectorVolume;
12            this.insertVolume = insertVolume;
13            this.bufferVolume = bufferVolume;
14            this.insertAmount = insertAmount;
15            this.vectorAmount = vectorAmount;
16        }
17    }
18    
19    public static LigationResult calculateLigationVolumes(
20            double vectorConcentration, double vectorLength, 
21            double insertConcentration, double insertLength,
22            double molarRatio, double totalVolume, double vectorAmount) {
23        
24        // 길이를 kb로 변환
25        double vectorLengthKb = vectorLength / 1000.0;
26        double insertLengthKb = insertLength / 1000.0;
27        
28        // 필요한 인서트 양 계산
29        double insertAmount = (vectorAmount * insertLengthKb / vectorLengthKb) * molarRatio;
30        
31        // 볼륨 계산
32        double vectorVolume = vectorAmount / vectorConcentration;
33        double insertVolume = insertAmount / insertConcentration;
34        double bufferVolume = totalVolume - vectorVolume - insertVolume;
35        
36        // 소수점 2자리로 반올림
37        vectorVolume = Math.round(vectorVolume * 100.0) / 100.0;
38        insertVolume = Math.round(insertVolume * 100.0) / 100.0;
39        bufferVolume = Math.round(bufferVolume * 100.0) / 100.0;
40        insertAmount = Math.round(insertAmount * 100.0) / 100.0;
41        
42        return new LigationResult(vectorVolume, insertVolume, bufferVolume, insertAmount, vectorAmount);
43    }
44    
45    public static void main(String[] args) {
46        LigationResult result = calculateLigationVolumes(50, 3000, 25, 1000, 3, 20, 50);
47        
48        System.out.printf("벡터: %.2f μL (%.2f ng)%n", result.vectorVolume, result.vectorAmount);
49        System.out.printf("인서트: %.2f μL (%.2f ng)%n", result.insertVolume, result.insertAmount);
50        System.out.printf("버퍼: %.2f μL%n", result.bufferVolume);
51        System.out.printf("총량: 20 μL%n");
52    }
53}
54

1#include <iostream>
2#include <cmath>
3#include <iomanip>
4
5struct LigationResult {
6    double vectorVolume;
7    double insertVolume;
8    double bufferVolume;
9    double insertAmount;
10    double vectorAmount;
11};
12
13LigationResult calculateLigationVolumes(
14    double vectorConcentration, double vectorLength,
15    double insertConcentration, double insertLength,
16    double molarRatio, double totalVolume, double vectorAmount = 50.0) {
17    
18    // 길이를 kb로 변환
19    double vectorLengthKb = vectorLength / 1000.0;
20    double insertLengthKb = insertLength / 1000.0;
21    
22    // 필요한 인서트 양 계산
23    double insertAmount = (vectorAmount * insertLengthKb / vectorLengthKb) * molarRatio;
24    
25    // 볼륨 계산
26    double vectorVolume = vectorAmount / vectorConcentration;
27    double insertVolume = insertAmount / insertConcentration;
28    double bufferVolume = totalVolume - vectorVolume - insertVolume;
29    
30    // 소수점 2자리로 반올림
31    vectorVolume = std::round(vectorVolume * 100.0) / 100.0;
32    insertVolume = std::round(insertVolume * 100.0) / 100.0;
33    bufferVolume = std::round(bufferVolume * 100.0) / 100.0;
34    insertAmount = std::round(insertAmount * 100.0) / 100.0;
35    
36    return {vectorVolume, insertVolume, bufferVolume, insertAmount, vectorAmount};
37}
38
39int main() {
40    LigationResult result = calculateLigationVolumes(50, 3000, 25, 1000, 3, 20);
41    
42    std::cout << std::fixed << std::setprecision(2);
43    std::cout << "벡터: " << result.vectorVolume << " μL (" << result.vectorAmount << " ng)" << std::endl;
44    std::cout << "인서트: " << result.insertVolume << " μL (" << result.insertAmount << " ng)" << std::endl;
45    std::cout << "버퍼: " << result.bufferVolume << " μL" << std::endl;
46    std::cout << "총량: 20 μL" << std::endl;
47    
48    return 0;
49}
50